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Image Search Results
Journal: Journal of translational medicine
Article Title: Cytosine-phosphate-guanine oligodeoxynucleotides alleviate radiation-induced kidney injury in cervical cancer by inhibiting DNA damage and oxidative stress through blockade of PARP1/XRCC1 axis.
doi: 10.1186/s12967-023-04548-y
Figure Lengend Snippet: Fig. 1 Protective effects of CpG-ODNs on RKI in relation to DNA damage and oxidative stress levels. A CpG-ODNs related sequence information; B Levels of blood urea nitrogen (BUN) and creatinine in mouse serum detected by automated biochemical analyzer; C Histopathological changes in mouse kidney tissues observed by HE staining (scale bar = 25 µm); D Levels of cellular apoptosis in mouse kidney tissues detected by TUNEL staining (scale bar = 25 µm), with brownish-gray indicating apoptotic cells; E Levels of DNA damage index (8-OHdG) in mouse serum detected by ELISA; F Levels of oxidative stress-related indexes in mouse serum detected by ELISA; G Levels of malondialdehyde (MDA) in mouse serum detected. * indicates P < 0.05 compared to the Normal group, # indicates P < 0.05 compared to the RKI group; each group consisted of 8 mice
Article Snippet: The levels of
Techniques: Sequencing, Staining, TUNEL Assay, Enzyme-linked Immunosorbent Assay
Journal: Journal of translational medicine
Article Title: Cytosine-phosphate-guanine oligodeoxynucleotides alleviate radiation-induced kidney injury in cervical cancer by inhibiting DNA damage and oxidative stress through blockade of PARP1/XRCC1 axis.
doi: 10.1186/s12967-023-04548-y
Figure Lengend Snippet: Fig. 5 Effect of CpG-ODNs on DNA damage and oxidative stress in HK-2 cells by regulating PARP1/XRCC1 axis. A CCK-8 assay for detection of viability of radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. B Caspase activity in radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1 as determined with enzymatic method. C Immunofluorescence assay to detect the γH2AX-positive cell ratio of radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. Blue indicated DAPI fluorescence labeled nucleus, and green indicated labeled γH2AX. D ELISA detection of levels of oxidative stress-related factors (SOD, CAT, GSH, GPx) in radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. E ELISA detection of MDA levels in radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. * P < 0.05 versus HK-2 cells treated with saline + oe-NC. # P < 0.05 versus HK-2 cells treated with CpG-ODN2006 + oe-NC. All cell experiments were repeated three times
Article Snippet: The levels of
Techniques: CCK-8 Assay, Activity Assay, Immunofluorescence, Fluorescence, Labeling, Enzyme-linked Immunosorbent Assay, Saline
Journal: Journal of translational medicine
Article Title: Cytosine-phosphate-guanine oligodeoxynucleotides alleviate radiation-induced kidney injury in cervical cancer by inhibiting DNA damage and oxidative stress through blockade of PARP1/XRCC1 axis.
doi: 10.1186/s12967-023-04548-y
Figure Lengend Snippet: Fig. 7 Schematic diagram of the molecular mechanism by which CpG-ODNs regulate PARP1/XRCC1 axis to affect DNA damage and oxidative stress response in RKI in cervical cancer. CpG-ODNs may attenuate RKI in cervical cancer by blocking PARP1/XRCC1 axis activation and inhibiting DNA damage and oxidative stress in renal tubular epithelial cells
Article Snippet: The levels of
Techniques: Blocking Assay, Activation Assay