new 8-ohdg check enzyme-linked immunosorbent assay kit (catalog Search Results


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Fig. 1 Protective effects of CpG-ODNs on RKI in relation to <t>DNA</t> <t>damage</t> and oxidative stress levels. A CpG-ODNs related sequence information; B Levels of blood urea nitrogen (BUN) and creatinine in mouse serum detected by automated biochemical analyzer; C Histopathological changes in mouse kidney tissues observed by HE staining (scale bar = 25 µm); D Levels of cellular apoptosis in mouse kidney tissues detected by TUNEL staining (scale bar = 25 µm), with brownish-gray indicating apoptotic cells; E Levels of DNA damage index (8-OHdG) in mouse serum detected by ELISA; F Levels of oxidative stress-related indexes in mouse serum detected by ELISA; G Levels of malondialdehyde (MDA) in mouse serum detected. * indicates P < 0.05 compared to the Normal group, # indicates P < 0.05 compared to the RKI group; each group consisted of 8 mice
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Fig. 1 Protective effects of CpG-ODNs on RKI in relation to <t>DNA</t> <t>damage</t> and oxidative stress levels. A CpG-ODNs related sequence information; B Levels of blood urea nitrogen (BUN) and creatinine in mouse serum detected by automated biochemical analyzer; C Histopathological changes in mouse kidney tissues observed by HE staining (scale bar = 25 µm); D Levels of cellular apoptosis in mouse kidney tissues detected by TUNEL staining (scale bar = 25 µm), with brownish-gray indicating apoptotic cells; E Levels of DNA damage index (8-OHdG) in mouse serum detected by ELISA; F Levels of oxidative stress-related indexes in mouse serum detected by ELISA; G Levels of malondialdehyde (MDA) in mouse serum detected. * indicates P < 0.05 compared to the Normal group, # indicates P < 0.05 compared to the RKI group; each group consisted of 8 mice
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Fig. 1 Protective effects of CpG-ODNs on RKI in relation to <t>DNA</t> <t>damage</t> and oxidative stress levels. A CpG-ODNs related sequence information; B Levels of blood urea nitrogen (BUN) and creatinine in mouse serum detected by automated biochemical analyzer; C Histopathological changes in mouse kidney tissues observed by HE staining (scale bar = 25 µm); D Levels of cellular apoptosis in mouse kidney tissues detected by TUNEL staining (scale bar = 25 µm), with brownish-gray indicating apoptotic cells; E Levels of DNA damage index (8-OHdG) in mouse serum detected by ELISA; F Levels of oxidative stress-related indexes in mouse serum detected by ELISA; G Levels of malondialdehyde (MDA) in mouse serum detected. * indicates P < 0.05 compared to the Normal group, # indicates P < 0.05 compared to the RKI group; each group consisted of 8 mice
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Fig. 1 Protective effects of CpG-ODNs on RKI in relation to <t>DNA</t> <t>damage</t> and oxidative stress levels. A CpG-ODNs related sequence information; B Levels of blood urea nitrogen (BUN) and creatinine in mouse serum detected by automated biochemical analyzer; C Histopathological changes in mouse kidney tissues observed by HE staining (scale bar = 25 µm); D Levels of cellular apoptosis in mouse kidney tissues detected by TUNEL staining (scale bar = 25 µm), with brownish-gray indicating apoptotic cells; E Levels of DNA damage index (8-OHdG) in mouse serum detected by ELISA; F Levels of oxidative stress-related indexes in mouse serum detected by ELISA; G Levels of malondialdehyde (MDA) in mouse serum detected. * indicates P < 0.05 compared to the Normal group, # indicates P < 0.05 compared to the RKI group; each group consisted of 8 mice
8 Ohdg Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 1 Protective effects of CpG-ODNs on RKI in relation to DNA damage and oxidative stress levels. A CpG-ODNs related sequence information; B Levels of blood urea nitrogen (BUN) and creatinine in mouse serum detected by automated biochemical analyzer; C Histopathological changes in mouse kidney tissues observed by HE staining (scale bar = 25 µm); D Levels of cellular apoptosis in mouse kidney tissues detected by TUNEL staining (scale bar = 25 µm), with brownish-gray indicating apoptotic cells; E Levels of DNA damage index (8-OHdG) in mouse serum detected by ELISA; F Levels of oxidative stress-related indexes in mouse serum detected by ELISA; G Levels of malondialdehyde (MDA) in mouse serum detected. * indicates P < 0.05 compared to the Normal group, # indicates P < 0.05 compared to the RKI group; each group consisted of 8 mice

Journal: Journal of translational medicine

Article Title: Cytosine-phosphate-guanine oligodeoxynucleotides alleviate radiation-induced kidney injury in cervical cancer by inhibiting DNA damage and oxidative stress through blockade of PARP1/XRCC1 axis.

doi: 10.1186/s12967-023-04548-y

Figure Lengend Snippet: Fig. 1 Protective effects of CpG-ODNs on RKI in relation to DNA damage and oxidative stress levels. A CpG-ODNs related sequence information; B Levels of blood urea nitrogen (BUN) and creatinine in mouse serum detected by automated biochemical analyzer; C Histopathological changes in mouse kidney tissues observed by HE staining (scale bar = 25 µm); D Levels of cellular apoptosis in mouse kidney tissues detected by TUNEL staining (scale bar = 25 µm), with brownish-gray indicating apoptotic cells; E Levels of DNA damage index (8-OHdG) in mouse serum detected by ELISA; F Levels of oxidative stress-related indexes in mouse serum detected by ELISA; G Levels of malondialdehyde (MDA) in mouse serum detected. * indicates P < 0.05 compared to the Normal group, # indicates P < 0.05 compared to the RKI group; each group consisted of 8 mice

Article Snippet: The levels of DNA damage index (8-OHdG) and each oxidative stress related factor in serum and cells were detected using mouse 8-OHdG (CSB-E10527m, Cusabio), human catalase (CAT) (JL14741, Shanghai Jianglai Biotechnology, Shanghai, China), mouse CAT (CSB-E14190m, Cusabio, Houston, TX), human superoxide dismutase (SOD) (CSB-E17064h, Cusabio), mouse SOD (JL12237, Shanghai Jianglai Biotechnology), human glutathione (GSH) (ml063305, Shanghai Meilian Biotechnology Co., Ltd.), mouse GSH (ml063305, Shanghai Meilian Biotechnology Co., Ltd.), mouse GSH (ml063305, Shanghai Meilian Biotechnology Co., Ltd.), human glutathione peroxidase (GPx) (JL10355, Shanghai Jianglai Biotechnology), mouse GPx (JL49904, Shanghai Jianglai Biotechnology) kits [39, 40].

Techniques: Sequencing, Staining, TUNEL Assay, Enzyme-linked Immunosorbent Assay

Fig. 5 Effect of CpG-ODNs on DNA damage and oxidative stress in HK-2 cells by regulating PARP1/XRCC1 axis. A CCK-8 assay for detection of viability of radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. B Caspase activity in radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1 as determined with enzymatic method. C Immunofluorescence assay to detect the γH2AX-positive cell ratio of radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. Blue indicated DAPI fluorescence labeled nucleus, and green indicated labeled γH2AX. D ELISA detection of levels of oxidative stress-related factors (SOD, CAT, GSH, GPx) in radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. E ELISA detection of MDA levels in radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. * P < 0.05 versus HK-2 cells treated with saline + oe-NC. # P < 0.05 versus HK-2 cells treated with CpG-ODN2006 + oe-NC. All cell experiments were repeated three times

Journal: Journal of translational medicine

Article Title: Cytosine-phosphate-guanine oligodeoxynucleotides alleviate radiation-induced kidney injury in cervical cancer by inhibiting DNA damage and oxidative stress through blockade of PARP1/XRCC1 axis.

doi: 10.1186/s12967-023-04548-y

Figure Lengend Snippet: Fig. 5 Effect of CpG-ODNs on DNA damage and oxidative stress in HK-2 cells by regulating PARP1/XRCC1 axis. A CCK-8 assay for detection of viability of radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. B Caspase activity in radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1 as determined with enzymatic method. C Immunofluorescence assay to detect the γH2AX-positive cell ratio of radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. Blue indicated DAPI fluorescence labeled nucleus, and green indicated labeled γH2AX. D ELISA detection of levels of oxidative stress-related factors (SOD, CAT, GSH, GPx) in radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. E ELISA detection of MDA levels in radiation-treated HK-2 cells in response to CpG-ODN2006 alone or combined with oe-PARP1 or oe-XRCC1. * P < 0.05 versus HK-2 cells treated with saline + oe-NC. # P < 0.05 versus HK-2 cells treated with CpG-ODN2006 + oe-NC. All cell experiments were repeated three times

Article Snippet: The levels of DNA damage index (8-OHdG) and each oxidative stress related factor in serum and cells were detected using mouse 8-OHdG (CSB-E10527m, Cusabio), human catalase (CAT) (JL14741, Shanghai Jianglai Biotechnology, Shanghai, China), mouse CAT (CSB-E14190m, Cusabio, Houston, TX), human superoxide dismutase (SOD) (CSB-E17064h, Cusabio), mouse SOD (JL12237, Shanghai Jianglai Biotechnology), human glutathione (GSH) (ml063305, Shanghai Meilian Biotechnology Co., Ltd.), mouse GSH (ml063305, Shanghai Meilian Biotechnology Co., Ltd.), mouse GSH (ml063305, Shanghai Meilian Biotechnology Co., Ltd.), human glutathione peroxidase (GPx) (JL10355, Shanghai Jianglai Biotechnology), mouse GPx (JL49904, Shanghai Jianglai Biotechnology) kits [39, 40].

Techniques: CCK-8 Assay, Activity Assay, Immunofluorescence, Fluorescence, Labeling, Enzyme-linked Immunosorbent Assay, Saline

Fig. 7 Schematic diagram of the molecular mechanism by which CpG-ODNs regulate PARP1/XRCC1 axis to affect DNA damage and oxidative stress response in RKI in cervical cancer. CpG-ODNs may attenuate RKI in cervical cancer by blocking PARP1/XRCC1 axis activation and inhibiting DNA damage and oxidative stress in renal tubular epithelial cells

Journal: Journal of translational medicine

Article Title: Cytosine-phosphate-guanine oligodeoxynucleotides alleviate radiation-induced kidney injury in cervical cancer by inhibiting DNA damage and oxidative stress through blockade of PARP1/XRCC1 axis.

doi: 10.1186/s12967-023-04548-y

Figure Lengend Snippet: Fig. 7 Schematic diagram of the molecular mechanism by which CpG-ODNs regulate PARP1/XRCC1 axis to affect DNA damage and oxidative stress response in RKI in cervical cancer. CpG-ODNs may attenuate RKI in cervical cancer by blocking PARP1/XRCC1 axis activation and inhibiting DNA damage and oxidative stress in renal tubular epithelial cells

Article Snippet: The levels of DNA damage index (8-OHdG) and each oxidative stress related factor in serum and cells were detected using mouse 8-OHdG (CSB-E10527m, Cusabio), human catalase (CAT) (JL14741, Shanghai Jianglai Biotechnology, Shanghai, China), mouse CAT (CSB-E14190m, Cusabio, Houston, TX), human superoxide dismutase (SOD) (CSB-E17064h, Cusabio), mouse SOD (JL12237, Shanghai Jianglai Biotechnology), human glutathione (GSH) (ml063305, Shanghai Meilian Biotechnology Co., Ltd.), mouse GSH (ml063305, Shanghai Meilian Biotechnology Co., Ltd.), mouse GSH (ml063305, Shanghai Meilian Biotechnology Co., Ltd.), human glutathione peroxidase (GPx) (JL10355, Shanghai Jianglai Biotechnology), mouse GPx (JL49904, Shanghai Jianglai Biotechnology) kits [39, 40].

Techniques: Blocking Assay, Activation Assay